NeuroVue® Dye Filter is useful for neuronal tract tracing studies of up to 3-4 weeks. Spectrally compatible with most fluorescent genetic tags, and NeuroVue Red, Orange & Emerald
Available in multiple colors, including far red, for multi-tract tracing and improved results even in tissues with high myelin expression
Applications:
NeuroVue Maroon has been found to be useful for tracing neuronal connections in animal tissues fixed in formaldehyde. Like other lipophilic tracers, it readily transfers into plasma membranes in fixed and/or live tissues and diffuses laterally within the membrane, eventually labeling the entire cell body as well as the finest axonal and dendritic branches, and allowing visualization of neuronal processes up to several millimeters distant from the point of dye insertion.
NeuroVue Maroon is provided in coated filter format because insertion of small dye coated filter segments has been shown to be a simple, reliable method for labeling well defined tissue regions, avoiding known artifacts associated with labeling via high pressure microinjection or insertion of dye crystals on a dissecting needle. NeuroVue Maroon fluoresces in the far red and exhibits minimal bleed through into filter windows typically used for visible fluorescing lipophilic tracers such as DiA, DiI, NeuroVue Red, NeuroVue Orange or NeuroVue Jade, making it an excellent choice for multicolor neurotracing studies in sections and/or whole-mount preparations.
Description: 1cm2 filter coated with the lipophilic far red emitting dye, NeuroVue® Maroon (ex max=647nm; em max=667nm).
References:
1. Beisel, K.W., Wang-Lundberg, Y., Maklad, A. and Fritzsch, B. 2005. Development and evolution of the vestibular sensory apparatus of the mammalian ear. J Vestibular Research 15:225-241. NeuroVue Maroon (previously PTIR271; see Hellard, Dev. Biol. 2004, below, for methods). de Caprona MD, Beisel KW, Nichols DH, Fritzsch B. 2004. Partial behavioral compensation is revealed in balance tasked mutant mice lacking otoconia. Brain Res Bull 64:289-301. Both NeuroVue Maroon (previously PTIR271) and NeuroVue Red (previously PTIR278) were used in Figure 8 (B. Fritzsch, personal communication). 3. Fritzsch, B, Nichols DH, Echelard Y, McMahon AP. 1995. Development of midbrain and anterior hindbrain ocular motoneurons in normal and Wnt-1 knockout mice, J Neurobiol. 27:457-469. Fritzsch B. 2003. Development of inner ear afferent connections: forming primary neurons and connecting them to the developing sensory epithelia. Brain Res Bull 60:423-433. NeuroVue Maroon (previously PTIR271) and NeuroVue Green (previously PTIR281) were used in Figure 4a, 4c,and 4d; NeuroVue Maroon and PKH26 were used for cover image (B. Fritzsch, personal communication) Fritzsch B, Tessarollo L, Coppola E, Reichardt LF. 2004. Neurotrophins in the ear: their roles in sensory neuron survival and fiber guidance. Prog Brain Res 146:265-278. NeuroVue Maroon (formerly PTIR271) and DiI were used in Figure 2 (B. Fritzsch, personal communication) Fritzsch B, Muirhead KA, Feng F, Gray BD, Ohlsson-Wilhelm BM. 2005. Diffusion and imaging properties of three new lipophilic tracers, NeuroVue Maroon, NeuroVue Red and NeuroVue Green and their use for double and triple labeling of neuronal profile. Brain Res Bull 66:249-258. NeuroVue Maroon, NeuroVue Red, NeuroVue Green Fritzsch B, Matei VA, Nichols DH, Bermingham N, Jones K, Beisel KW, Wang VY. 2005. Atoh1 null mutants show directed afferent fiber growth to undifferentiated ear sensory epithelia followed by incomplete fiber retention. Dev Dyn, 233: 570-583. NeuroVue Maroon (previously PTIR271), NeuroVue Red (previously PTIR278) Fritzsch B, Pauley S, Matei V, Katz DM, Xiang M, Tessarollo L. 2005. Mutant mice reveal the molecular and cellular basis for specific sensory connections to inner ear epithelia and primary nuclei of the brain. Hear Res, 206: 52-63. NeuroVue Maroon (previously PTIR271); see Hellard, Dev Biol 2004, below, for methods) Fritzsch B, Jackson Lab Presentation, 2005: http://www.biomedsci.creighton.edu/facilities/nccb/media/Jackson_lab_presentation.ppt NeuroVue Green (previously PTIR281);NeuroVue Red (previously PTIR278); NeuroVue Maroon (previously PTIR271) Gu C, Rodriguez ER, Reimert DV, Shu T, Fritzsch B, Richards LJ, Kolodkin AL, Ginty DD. 2003. Neuropilin-1 Conveys Semaphorin and VEGF Signaling during Neural and Cardiovascular Development. Dev Cell 5:45-57. NeuroVue Maroon (formerly PTIR271) rather than PKH26 was used in Figure 3 (B. Fritzsch, personal communication) Gurung B, Fritzsch B. 2004. Time course of embryonic midbrain and thalamic auditory connection development in mice as revealed by carbocyanine dye tracing. J Comp Neurol 479:309-327. NeuroVue Maroon (previously PTIR271), NeuroVue Red (previously PTIR278) Hellard D, Brosenitsch T, Fritzsch B, Katz DM. 2004. Cranial sensory neuron development in the absence of brain-derived neurotrophic factor in BDNF/Bax double null mice. Dev Biol 275:34-43. NeuroVue Maroon (previously PTIR271)) Honig M. DiI Labelling. 1993. Neuroscience Protocols 93-050-16-01-20 Hsieh CY, Cramer KS. 2006. Deafferentation Induces Novel Axonal Projections in the Auditory Brainstem After Hearing Onset. J Comp Neurol 497: 589-599 NeuroVue Red was used for all figures except Figure 2D, for which both NeuroVue Red and DiI were used, and Figure 5A, for which DiI was used (K. Cramer, personal communication).
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