NeuroVue® Dye Filter is useful for neuronal tract tracing studies of up to 3-4 weeks. Only recommended for tract tracing studies of up to 48 hours. Spectrally compatible with NeuroVue Maroon, Orange & Red.
Available in multiple colors, including far red, for multi-tract tracing and improved results even in tissues with high myelin expression
Applications:
NeuroVue Emerald is an analog of NeuroVue Green (1, 2) with optimized diffusion properties for studies in formaldehyde fixed small embryos of up to 48h (at longer time periods transcellular labeling becomes substantial). For these types of short term studies in fixed tissue, NeuroVue Jade can be applied simultaneously in combination with NeuroVue Maroon, NeuroVue Red and/or NeuroVue Orange to provide 3-4 color tracing of neuronal connections (personal communication, B. Fritzsch, Creighton University). Like other lipophilic tracers (3, 4), it readily transfers into plasma membranes in fixed tissues and diffuses laterally within the membrane, eventually labeling the entire cell body as well as the finest axonal and dendritic branches, and allowing visualization of neuronal processes up to several millimeters distant from the point of dye insertion (1, 2).
NeuroVue® Jade is provided in coated filter format because insertion of small dye coated filter segments has been shown to be a simple, reliable method for labeling well defined tissue regions, avoiding known artifacts associated with labeling via high pressure microinjection or insertion of dye crystals on a dissecting needle (3, 5, 6). NeuroVue® Jade fluoresces in the green region of the spectrum and exhibits minimal bleed through into filter windows typically used for the visible fluorescing lipophilic tracers, DiI, NeuroVue Red, NeuroVue Orange and also the far red fluorescing NeuroVue Maroon and NeuroVue Burgundy, making it an excellent choice for multicolor neurotracing studies in sections and/or whole-mount preparations (1, 2) for periods of up to 48 hours.
Description: 1cm² nylon filter coated with the lipophilic green emitting dye, NeuroVue Jade. Typical dye loading: 22-30nmoles/mm2
References:
1. Fritzsch B, Muirhead KA , Feng F , Gray BD, Ohlsson-Wilhelm BM. 2005. Diffusion and Imaging Properties of three new lipophilic tracers, NeuroVue (TM) Maroon, NeuroVue(TM) Red and NeuroVue(TM) Green and their use for double and triple labeling of neuronal profile. Brain Res Bull., 66, 3, 249-258
2. Fritzsch B, Jackson Lab Presentation, 2005: http://www.biomedsci.creighton.edu/facilities/nccb/media/Jackson_lab_presentation.ppt NeuroVue Green (previously PTIR281);NeuroVue Red (previously PTIR278);NeuroVue Maroon (previously PTIR271)
3. Honig M. 1993 DiI Labelling. Neuroscience Protocols 93-050-16-01-20 .
4. Köbbert C, Apps R, Bechmann I, Lanciego JL, Mey J,Thanos S. 2000. Current concepts of neuroanatomical tracing. Progress in Neurobiology 62: 327-351.
5. Fritzsch, B, Nichols DH, Echelard Y, McMahon AP. 1995. Development of midbrain and anterior hindbrain ocular motoneurons in normal and Wnt-1 knockout mice, J Neurobiol. 27:457-469.
6. Rosa-Molinar E, Proskocil BJ, Ettel M and Fritzsch B. 1999. Whole-mount procedures for simultaneous visualization of nerves, neurons, cartilage and bone. Brain Res. Protoc. 4, 115-123.
Sold under sublicense from PTI Research, Inc. to MTTI . NeuroVue® is a trademark of PTI Research, Inc.