Starter Kit Contains:

• 10 transportation vials filled with 5 ml HOPE® I with protection vials

• 1 x 1 ml HOPE® II

• 200 grams low melt point paraffin

Benefits:

• Save Time - Perform IHC on paraffin embedded tissue without further antigen retrieval steps, saving 20 minutes  to 1 hour.

• Compatibility - Allows the use of antibodies that are usually not recommended or intended for formalin fixed paraffin embedded tissue.

• Problem Free - No more overfixed formalin tissue samples.

• Outstanding Performance - Unmatched results in RNA and DNA in-situ hybridization.

• Effective - Excellent conservation of nucleic acids for PCR and RT-PCR.

Reveal Fascinating Morphology using the New HOPE®-Fixation Technique

Achieve multiple applications including immunohistochemistry without antigen unmasking on paraffin embedded tissue

HOPE® = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect. Hope is a combination of amino acids and glucose blended together with an organic buffer. Dehydration by acetone as the single dehydrating agent and pure paraffin denature proteins and preserve nucleic acids and antigenic-structures allowing clearly visible molecular level results.

Polysciences, Inc. is pleased to announce a new fixation-technique for cells and tissues utilizing the revolutionary HOPE®-fixative. Scientists have developed an exciting new fixative, which has started to greatly enhance the possibilities of tissue paraffin-embedded archival materials. The HOPE® technique is mainly used for the preparation of paraffin embedded tissue sections. In contrast to other fixation methods HOPE® does not completely denature or cross-link structural proteins, enzymes, and nucleic acids. Applications for immunohistochemistry, in situ hybridization targeting RNA and DNA, PCR, and RT-PCR have been demonstrated using properly prepared paraffin embedded tissue sections. Tissue specimens fixed with the HOPE technique allow greater morphology and retrospective studies on the molecular level.

Hope Fixation References:
Basics
Olert J, Wiedorn KH, Goldmann T, Kühl H, Mehraein Y, Scherthan H, Niketeghad F, Vollmer E, Müller AM, Müller-Navia J (2001) HOPE-fixation: A novel fixing method and paraffin embedding technique for human soft tissues. PATHOLOGY RESEARCH AND PRACTICE 197: 823-826

Immunohistochemistry
Goldmann T, Dromann D, Marzouki M, Schimmel U, Debel K, Branscheid D, Zeiser T, Rupp J, Gerdes J, Zabel P, Vollmer (2005) Tissue microarrays from HOPE-fixed specimens allow for enhanced high throughput molecular analyses in paraffin-embedded material. PATHOL RES PRACT. 201(8-9): 599-602

Goldmann T, Vollmer E, Gerdes J (2003) What’s cooking? Detection of important biomarkers in HOPE-fixed, paraffin-embedded tissues eliminates the need for antigen retrieval. AMERICAN JOURNAL OF PATHOLOGY 163: 2638-2640,

Olert J, Wiedorn KH, Goldmann T, Kühl H, Mehraein Y, Scherthan H, Niketeghad F, Vollmer E, Müller AM, Müller-Navia J (2001) HOPE-fixation: A novel fixing method and paraffin embedding technique for human soft tissues. PATHOLOGY RESEARCH AND PRACTICE 197: 823-826

Pictures kindly provided by Dr. T Goldman & Prof. Dr. E Vollmer, Research Center Borstel, D 23845 Borstel, Germany
HOPE® is a registered trade mark of DCS, Innovative Diagnostik Systeme, Germany. HOPE® is manufactured by DCS, Germany.