Product Description:
A far-red emitting dye, belonging to the squaraine rotaxane family of dyes, which bears a free carboxyl group that can be coupled to free amino groups. Spectral properties: abs max = 650 nm and emission max = 678 nm. Provided in Crystalline Powder form.
What are SRfluor™ dyes?
SRfluor™ dyes belong to the family of squaraine rotaxane dyes which exhibit absorption and emission properties in the far-red region of the spectrum.
What are the advantages to SRfluor™ dyes?
- Improved chemical and photochemical stability compared with squaraines, cyanines, Alexa® and Atto dyes.
- Exhibit sharp absorption and emission bands thus provide reduced excitation and emission cross talk into channels where other fluorochromes are being used.
- Do not exhibit aggregation induced spectral broadening under biological conditions at micromolar concentrations.
- 5-20X brighter compared with cyanines, Alexa® and Atto dyes.
- Photophysical properties stable over the pH range from 2-12.
- Spectral properties compatible with the 633nm or 647nm laser lines on flow cytometers, confocal and in vivo imaging equipment.
Product size: 1 mg of crystalline dark green powder.
Molecular Weight: 1109
Product Purity: >90% by HPLC
Extinction Coefficient (DMSO): 242,500 cm-1 M-1 (650 nm).
Storage/Stability: Solid should be stored in the dark at room temperature. Stock solutions should be stored in the dark at 0-4 °C and are found to be stable for at least 1 month.
Applications: SRfluor™-680 Carboxylate has been found to be 5-20X brighter compared with cyanines, Alexa® and Atto dyes and also have improved chemical and photochemical stability (1-3). It can be coupled to peptides, proteins and antibodies, etc., to provide fluorescent conjugates for use in Western-Blots and in vivo imaging studies.
Additional Information
• 1mg of SRfluor™-680 Carboxylate can be dissolved in 0.9mL of DMF/DMSO to provide a 1mM stock solution.
• SRfluor™-680 Carboxylate can be efficiently excited with either 633nm or 647nm laser lines and detected using a standard filter set-up for Cy5.
References:
(1) Johnson, J. R.; Fu, N.; Arunkumar, E.; Leevy, W. M.; Gammon, S. T.; Piwinica-Worms, D.; Smith, B. D. Squaraine Rotaxanes: Superior Substitutes for Cy-5 in Molecular Probes for Near-Infrared Fluorescence Cell Imaging Angew. Chem. Int. Ed. 2007, 46, 5528.
(2) Arunkumar, E.; Fu, N.; Smith, B. D. Squaraine-Derived Rotaxanes: Highly Stable, Fluorescent Near-IR Dyes. Chem.-Eur. J. 2006, 12, 4684.
(3) Arunkumar, E; Forbes, C. C.; Noll, B. C.; Smith B. D. Squaraine-Derived Rotaxanes: Sterically Protected Fluorescent Near-IR Dyes J. Am. Chem. Soc. 2005, 127, 3288.
SRfluorTM is a trademark of Molecular Targeting Technologies, Inc.
Distributed by Polysciences, Inc. under agreement from MTTI.