Trypan blue, C.I. 23850
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Trypan blue, C.I. 23850
CAS Number: 72-57-1
(Direct blue 14; Niagara blue 3B)
Trypan blue has been widely used to assess the viability of eukaryotic cells. This method is often called trypan blue exclusion. Nonviable cells will exhibit a marked concentration of this blue dye in their nuclei. Tissue culture labs will find it extremely useful for determining the health and density of cell lines. Other studies have demonstrated trypan blue as an inactivating agent for measles and herpes simplex viruses and as a teratogenic agent in rats.
λ max 607nm
Molecular Weight: 960.83
Melting Point: >300°
References: J. Histochem. Cytochem., 18, 581 (1970); J. Gen. Virol., 64, 1365 (1983); Teratology, 26, 289 (1982)
Hazards:
Irritant,
Toxic (Ingestion),
Toxic (Absorption),
Toxic (Inhalation)
Handling: Glove, chemical goggles & fume hood
Storage: Store at room temperature
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