Bisbenzimide (Hoechst 33258)
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Bisbenzimide (Hoechst 33258)
CAS Number: 23491-45-4
Fluorescent chromosome stain. Recommended use is 10mg/ml for 2-10 minutes. This will vary based on section thickness.
(2’-[4-Hydroxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride pentahydrate; Hoechst 33258)
Molecular Weight: 623.97
Photo: Rat Brain Sagittal, 8 micrometer section, stained with Hoechst 33342, Alexa Fluor 568-6FAP (Rb) and Alexa Fluor 488-NF-P (Ms). Photo: Mike Davidson of Florida State University
References: Science, 220, 620 (1983), Anal. Biochem., 179, 401 (1989)
Hazards:
Irritant
Handling: Gloves & chemical goggles
Storage: Protect from light, Protect from moisture, Store at 4 degrees celsius
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