Useful for tract tracing studies of up to 7 days. Spectrally compatible with most fluorescent genetic tags and NeuroVue Red, Orange, Jade. Spectral unmixing required for use with NeuroVue Maroon.
Advantages:
- Convenient, ready-to-use coated filter format More precise control of dye insertion point
- No messy oils, pastes or hard-to-position crystals
- Diffusion properties comparable to or better than other commercially available neurotracing dyes
- More focal results (e.g. labeling of small sets of axons within a pathway)
- Available in multiple colors, including far red, for multi-tract tracing and improved results even in tissues with high myelin expression
Applications
NeuroVue® Burgundy has been found to be useful for tracing neuronal connections in animal tissues fixed in formaldehyde (personal communication, Dr Bernd Fritzsch, Creighton University). Like other lipophilic tracers (1, 2), it readily transfers into plasma membranes in fixed and/or live tissues and diffuses laterally within the membrane, eventually labeling the entire cell body as well as the finest axonal and dendritic branches, and allowing visualization of neuronal processes up to several millimeters distant from the point of dye insertion. Studies of up to 7 days can be performed in most cases without substantial transcellular diffusion.
NeuroVue® Burgundy is provided in coated filter format because insertion of small dye coated filter segments has been shown to be a simple, reliable method for labeling well defined tissue regions, avoiding known artifacts associated with labeling via high pressure microinjection or insertion of dye crystals on a dissecting needle (1, 3, 4). NeuroVue Burgundy fluoresces in the far red and exhibits minimal bleed through into filter windows typically used for visible fluorescing lipophilic tracers such as DiA, DiI, NeuroVue Red, NeuroVue Orange or NeuroVue Jade, making it an excellent choice for multicolor neurotracing studies in sections and/or whole-mount preparations. NeuroVue Burgundy can also be used in combination with NeuroVue Maroon using spectral unmixing techniques.
Additional Information
Filter segments of the desired size and shape can be cut using super fine Vannas scissors and inserted into the tissue at the site to be labeled.
Diffusion times vary depending on the biological system under study and must be determined empirically.
Detection of Labeled Cells
Note: Due to its very long red fluorescence emission, most people cannot see NeuroVue® Burgundy emission by eye. Detection by camera will be more sensitive than with the unaided eye.
Confocal microscopy:
Detection is most efficient using the 633nm or 647nm laser line for excitation and emission filter set at 650-710nm.
Epifluorescence microscopy:
Standard filter sets potentially useful for NeuroVue Burgundy excitation and emission include: Cy5® exciter D640/20x, dichroic 660DCLP, emitter D680/30 Cy5® longpass emission, exciter HQ620/60x, dichroic Q660LP, emitter HQ665LP
Description
1cm² filter coated with lipophilic far red emitting dye, NeuroVue® Burgundy (ex max=683nm; em max=707nm).
Recent Publications:
Diffusion and Imaging Properties of three new lipophilic tracers, NeuroVue® Maroon, NeuroVue® Red and NeuroVue® Green and their use for double and triple labeling of neuronal profile. B Fritzsch et al. Brain Res Bull., 66, 3, 249-258 (2005).
A disorganized innervation of the inner ear persists in the absence of ErbB2. JK Morris et al. Brain Res.,1091:186-199 (2006).
Deafferentiation induces novel projections in the auditory brainstem after hearing onset. CY Hsieh and KS Cramer. Journal of Comparative Neurology, 497:589-599 (2006)
Al-Mehdi AB, Patel M, Haroon A, Reed D, Ohlsson-Wilhelm B, Muirhead K and Gray BD. Increased Depth of Cellular Imaging in the Intact Lung using Far Red and Near Infrared Fluorescent Probes. Int. J. Biomed Imaging. 1-7 (2006).
Stewart CC, Woodring ML, Podniesinski E and Gray BD. Flow cytometer in the infrared: inexpensive modifications to a commercial instrument. Cytometry, Part A, 67A, #2, 104-111, 2005
Sold under sublicense from PTI Research, Inc. to MTTI . NeuroVue® is a trademark of PTI Research, Inc.