Super Decal II – Heavy Duty Decal is designed for safe, longer term decalcification of hard compact bone, femoral heads, above and below the knee amputation - AKA, BKA.
- Save Time - Decalcify in as little as 3 hours!
- Ready-to-use decalcifier for human and animal tissues
- Enhanced nuclear detail
- Environmentally safe and biodegradable
Gold Standard Series: Super Decal II – Heavy Duty Decal is an extremely effective and versatile decalcifier that can be used in specific lab routines. As with most acids, nuclei acids in the cell can become subject to ribonuclease digestion, resulting in a loss of basophilic properties. Careful monitoring should be used with Super Decal II – Heavy Duty Decal to avoid over decalcification. Most decalcification occurs in approximately 4 - 6 hours or less, depending on the thickness and density of the specimens. Overnight decalcification should be avoided. Super Decal II – Heavy Duty Decal is not recommended for IHC.
Reducing temperature of the decalcifying solution to approximately 20 degrees centigrade will promote histochemical staining in procedures such as H & E, Masson’s, Van Gieson’s and Azure-Eosin.
Rinse specimens thoroughly in running tap water after decalcification. Do not mix with formalin and avoid contact with metal forceps and metal surfaces. Wear gloves and protective clothing during decalcification procedures. Perform procedures under a fume hood. Store decal specimen in 70% ethyl alcohol until ready to process.
| Bone Sample |
Suggested Time |
| Bone Marrow Biopsies |
15 – 30 Minutes |
| Small Cancellous Bone |
2 – 4 Hours |
| Femur Wedge |
3 – 5 Hours |
| Mature Bone (1 cm) |
4 – 6 Hours |
| Whole Femur or Teeth |
Overnight |
Photo of Bottlenose Dolphin calf teeth courtesy of Rachel Neuenhoff and Christopher D. Marshall, Ph.D., Department of Marine Biology, Texas A&M University, Galveston, TX.