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Fluorescein isothiocyanate, Certified, FITC
CAS Number: 3326-32-7
Reagent widely used for fluorescent labeling of proteins. Also used in the fluorescent antibody technique for identification of pathogens.
Fluorescein 5-isothiocyanate; FITC
λmax 499nm
isomer I, 90% minimum
laser grade
Molecular Weight: 389.4
Melting Point: >360°
Photo: Rat dorsal ganglion, triple-labeled with FITC (CGRP) secondary antibodies, Texas Red® (extracellular matrix) and avidin-Pacific Blue (biotinylated isolectin GS-I-B4). Photo: Jeffrey C. Petruska, Dept. of Neuroscience, and Richard D. Johnson, PhD, Dept. of Physiological Science and Neuroscience, University of Florida Brain Institute.
References: Am. J. Pathol., 34, 1081 (1958); Methods in Enzymology, 26, 28 (1972); Anal. Biochem., 57, 227 (1974)
Hazards:
Irritant,
Poison (Ingestion),
Poison (Absorption),
Poison (Inhalation)
Handling: Gloves & fume hood
Storage: Store below 0 degrees celsius