PAS techniques are used to demonstrate polysaccharides, neutral mucosubstances and basement membranes primarily in tissue. The PAS reagent is also called Fuelgen Stain for the demonstration of DNA with a different protocol. Kidney is the most sensitive control. The demonstration of glycogen is best represented by a section of liver with a digestion step used as a negative control in the staining.
The kit is supplied with Harris Hematoxylin Acidified as the counterstain.
- 0.5% Periodic Acid Aq (250 ml)
- Schiff’ Reagent (250 ml)
- 0.55% Potassium Metabisulfite (250 ml)
- Harris Hematoxylin Acidified (250 ml)
- Phosphate Citrate Buffer pH 5.0 (250 ml)
- Diastase Powder (0.5 gm)