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4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI)
CAS Number: 28718-90-3
A cationic fluorescent dye which specifically binds to adenine-thymine-rich DNA. Applications include detection of nanogram quantities of DNA in cellular homogenates, and cytofluorometric determination of the DNA base content in human chromosomes. Available in bulk quantities for OEM users at significant savings.
λ max 342nm
Molecular Weight: 350.25
Photo: Mouse Kidney cells were stained with DAPI, Alexa Fluor® 488 wheat germ agglutinin, Alexa Fluor® 568phalloidin. Contributed by Walt Metcalfe, Molecular Probes, Inc.; photographed by Gregg Jarvis, Omega Optical, Inc.
References: Nature, 253, 461 (1975); Anal. Biochem., 92, 497 (1979); Stain Technol., 60, 7 (1985); Eur. J. Biochem., 182, 437 (1989)
Storage: Protect from light, Handle under dry nitrogen, Store below -20 degrees celsius