Secondary antibodies and conjugates, affinity purified
We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjuagte for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions. Products without specifically stated dilutions should be used according to the information below.
| Conjugate |
ELISA |
Blotting |
Flow |
Tissue |
|
| Flourescein |
nd |
nd |
1:50-1:200 |
1:200-1:1000 |
| Rhodamine, Texas Red |
nd |
nd |
1:50-1:200 |
1:200-1:1000 |
| Biotin |
1:5k-1:20k |
1:3k-1:10k |
nd |
1:100-1:1000 |
| Peroxidase |
1:40k-1:10k |
1:2k-1:5k |
nd |
1:200-1:2000 |
| Alkaline phosphatase |
1:1k-1:2k |
1:500-1:1k |
nd |
1:100-1:1000 |
(minimum cross-reactivity to Ch, GP, Ham, Hs, Hu, Ms, Rb & Rt serum proteins), alkaline phosphatase conjugate, liquid