AFB Ziehl-Neelson Kit (Hot Method)
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AFB Ziehl-Neelson Kit (Hot Method)
Bacteriological stain kit used to identify acid-fast organisms, mainly Mycobacteria. Helpful in diagnosing Mycobacterium tuberculosis since its lipid rich cell wall makes it resistant to Gram stain.
Can also be used to stain other bacteria like Nocardia. Kit contains a primary dye (carbol-fuchsin), a decolorizer (acid-alcohol) and a counter stain (methylene blue). Decolorizer is used to decolorize the bacteria that "dislike" the primary stain. Counter stain is used to stain those bacteria which were decolorized by the acid alcohol. Acid-fast bacilli will stain bright red, while non-acid fast organisms will stain blue or green.
Kit includes: Carbol Fuchsin (Ziehl-Neelson), Acid Alcohol, and Methylene Blue (8 oz. bottle of each)
Photo: Mycobacterium tuberculosis bacteria revealed using acid-fast Ziehl- Neelson stain; Magnified 1000X. Image courtesy of CDC Publich Health Library/Dr. George P. Kubica.
Hazards:
Flammable liquid,
Irritant
Handling: Glove, chemical goggles & fume hood
Storage: Store at room temperature
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