AFB Kinyoun Kit (Cold Method)
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AFB Kinyoun Kit (Cold Method)
Method of staining acid-fast microorganisms, specifically mycobacterium. Procedure is similar to Ziehl-Neelson stain, but does not involve heating the slides.
Kinyoun staining method uses carbol-fuchsin as a primary stain, followed by decolorization with an acid-alcohol solution and methylene blue as a counterstain. Kinyoun carbol-fuschsin has a greater concentration of phenol and basic fuchsin and does not require heating in order to stain properly.
When viewed under a microscope, Kinyoun stained slides will show acid-fast organisms as red and non acid-fast organisms as blue.
Kit includes: Carbol Fuchsin (Kinyoun), Acid Alcohol, and Methylene Blue (8 oz. bottle of each)
Photo: Direct fecal smear is stained to detect Cryptosporidium sp., an intracellular protozoan parasite using a modified cold Kinyoun acid-fast staining technique. Image courtesy of CDC Public Health Library
Hazards:
Flammable liquid,
Irritant,
Toxic (Ingestion),
Toxic (Absorption),
Toxic (Inhalation)
Handling: Glove, chemical goggles & chemical mask or hood
Storage: Store at room temperature
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