AFB Kinyoun Kit
AFB Kinyoun Kit
Although no definative evidence has been presented, the high lipid content (especially the mycolic acid component ) of mycobacteria is thought to be related to the mechanism of acid fastness. It has long been known that even light mechanical injury to the cell wall will cause them to lose the acid fast characteristic, suggesting that permeation through cell membranes might be an important part of the mechanism. Carbol fuchsin is used to stain the slide. Acid alcohol is used to decolorize the slide. It has been sugested that the dye replacement power of the counter stain could be used to decolorize and counter stain at the same time. It has been shown that acid alcohol increases the differentiation obtained, and gives superior results. After decolorization, the slide may be counter stained with methylene blue or brilliant green. The acid fast organisms will appear red while non-acid fast organisms will stain blue or green.
AFB Kinyoun Kit includes one 8 oz. bottle of each of the following:
Carbol Fuchsin (Kinyoun)
Acid Alcohol
Methylene Blue
Photo: This micrograph of a direct fecal smear is stained to detect Cryptosporidium sp., an intracellular protozoan parasite using a modified cold Kinyoun acid-fast staining technique. Image courtesy of CDC Public Health Library/ J. of Inf. Dis., Vol. 137, #5, pp. 824-828, May 1983, and Dr. Pearl Ma, Dir., Microbiology, St. Vincent's Hosp., N .Y.
Hazards:
Flammable liquid,
Irritant,
Toxic (Ingestion),
Toxic (Absorption),
Toxic (Inhalation)
Handling: Glove, chemical goggles & chemical mask or hood
Storage: Store at room temperature
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