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AFB Kinyoun Kit (Cold Method)

AFB Kinyoun Kit (Cold Method)

Method of staining acid-fast microorganisms, specifically mycobacterium. Procedure is similar to Ziehl-Neelson stain, but does not involve heating the slides.

Kinyoun staining method uses carbol-fuchsin as a primary stain, followed by decolorization with an acid-alcohol solution and methylene blue as a counterstain. Kinyoun carbol-fuschsin has a greater concentration of phenol and basic fuchsin and does not require heating in order to stain properly.

When viewed under a microscope, Kinyoun stained slides will show acid-fast organisms as red and non acid-fast organisms as blue.

AFB Kinyoun Kit includes one 8 oz. bottle each of the following:

 

  • Carbol Fuchsin (Kinyoun)
  • Acid Alcohol
  • Methylene Blue

    Photo: micrograph of a direct fecal smear stained to detect Cryptosporidium sp., an intracellular protozoan parasite using a modified cold Kinyoun acid-fast staining technique.
    Image courtesy: CDC Public Health Library/ J. of Inf. Dis., Vol. 137, #5, pp. 824-828, May 1983, and Dr. Pearl Ma, Dir., Microbiology, St. Vincent's Hosp., N .Y.

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    Hazards: Caution!Flammable liquid, Caution!Irritant, Warning!Toxic (Ingestion), Warning!Toxic (Absorption), Warning!Toxic (Inhalation)
    Handling: Glove, chemical goggles & chemical mask or hood
    Storage: Store at room temperature

    MSDS / Technical Data Sheets

    Please select a Continent for pricing information.
    Catalog No. Packaging Size Price Quantity
    24670-1
    Size:  1 kit