AFB Kinyoun Kit (Cold Method)
AFB Kinyoun Kit (Cold Method)
Method of staining acid-fast microorganisms, specifically mycobacterium. Procedure is similar to Ziehl-Neelson stain, but does not involve heating the slides.
Kinyoun staining method uses carbol-fuchsin as a primary stain, followed by decolorization with an acid-alcohol solution and methylene blue as a counterstain. Kinyoun carbol-fuschsin has a greater concentration of phenol and basic fuchsin and does not require heating in order to stain properly.
When viewed under a microscope, Kinyoun stained slides will show acid-fast organisms as red and non acid-fast organisms as blue.
AFB Kinyoun Kit includes one 8 oz. bottle each of the following:
Carbol Fuchsin (Kinyoun)
Acid Alcohol
Methylene Blue
Photo: micrograph of a direct fecal smear stained to detect Cryptosporidium sp., an intracellular protozoan parasite using a modified cold Kinyoun acid-fast staining technique.
Image courtesy: CDC Public Health Library/ J. of Inf. Dis., Vol. 137, #5, pp. 824-828, May 1983, and Dr. Pearl Ma, Dir., Microbiology, St. Vincent's Hosp., N .Y.
Hazards:
Flammable liquid,
Irritant,
Toxic (Ingestion),
Toxic (Absorption),
Toxic (Inhalation)
Handling: Glove, chemical goggles & chemical mask or hood
Storage: Store at room temperature
Please select a Continent for pricing information.