HOPE® Fixative System I

Formalin free fixation technique for molecular pathology and research

Reveal Fascinating Morphology using the HOPE^®-Fixation Technique!

Achieve multiple applications including immunohistochemistry without antigen unmasking on paraffin embedded tissue

HOPE^® = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect. Hope is a combination of amino acids and glucose blended together with an organic buffer. Dehydration by acetone as the single dehydrating agent and pure paraffin denature proteins and preserve nucleic acids and antigenic-structures allowing clearly visible molecular level results.

Applications:

• PCR
• RT-PCR
• Immunohistochemistry
• In situ hybridization targeting RNA and DNA

Benefits:

• Achieve IHC on paraffin tissue without antigen unmasking
• IHC using antibodies which do not work on formalin fixed parafffin embedded tissue
• No more overfixed formalin tissue samples
• Unmatched results in RNA and DNA in situ hybridization
• Excellent conservation of nucleic acids for PCR and RT-PCR

New fixation-technique for cells and tissues utilizing the revolutionary HOPE®-fixative. Scientists have developed an exciting new fixative, which has started to greatly enhance the possibilities of tissue paraffin-embedded archival materials.

The HOPE^® technique is mainly used for the preparation of paraffin embedded tissue sections. In contrast to other fixation methods HOPE^® does not completely denature or cross-link structural proteins, enzymes, and nucleic acids.

Applications for immunohistochemistry, in situ hybridization targeting RNA and DNA, PCR and RT-PCR have been demonstrated using properly prepared paraffin embedded tissue sections. Tissue specimens fixed with the HOPE technique allow greater morphology and retrospective studies on the molecular level.

The HOPE®-Fixation technique requires HOPE^® Fixative System I and HOPE^® Fixative System II to complete the protocol.

See Catalog #24824 for Hope^® Fixative System II

Hope Fixation References:
Basics
Olert J, Wiedorn KH, Goldmann T, Kühl H, Mehraein Y, Scherthan H, Niketeghad F, Vollmer E, Müller AM, Müller-Navia J (2001) HOPE-fixation: A novel fixing method and paraffin embedding technique for human soft tissues. PATHOLOGY RESEARCH AND PRACTICE 197: 823-826

Immunohistochemistry
Goldmann T, Dromann D, Marzouki M, Schimmel U, Debel K, Branscheid D, Zeiser T, Rupp J, Gerdes J, Zabel P, Vollmer (2005) Tissue microarrays from HOPE-fixed specimens allow for enhanced high throughput molecular analyses in paraffin-embedded material. PATHOL RES PRACT. 201(8-9): 599-602

Goldmann T, Vollmer E, Gerdes J (2003) What’s cooking? Detection of important biomarkers in HOPE-fixed, paraffin-embedded tissues eliminates the need for antigen retrieval. AMERICAN JOURNAL OF PATHOLOGY 163: 2638-2640,

Olert J, Wiedorn KH, Goldmann T, Kühl H, Mehraein Y, Scherthan H, Niketeghad F, Vollmer E, Müller AM, Müller-Navia J (2001) HOPE-fixation: A novel fixing method and paraffin embedding technique for human soft tissues. PATHOLOGY RESEARCH AND PRACTICE 197: 823-826

Pictures kindly provided by Dr. T Goldman & Prof. Dr. E Vollmer, Research Center Borstel, D 23845 Borstel, Germany
HOPE® is a registered trade mark of DCS, Innovative Diagnostik Systeme, Germany. HOPE® is manufactured by DCS, Germany.