Purolite® ECR8404F Amino C6 Methacrylate is an amino resin with low porosity. This carrier is stable during storage and can be easily handled before and after immobilization procedures. ECR8404F Amino C6 Methacrylate requires pre-activation by glutaraldehyde and then can be used in the covalent immobilization of enzymes. Reaction of the aldehyde groups with amino groups of enzymes to form Schiff bases is fast and gives stable multipoint covalent binding with mainly ε-amino groups of Lysines. An even more stable linkage can be achieved by reduction with borohydrides. Immobilisation proceeds at low ionic buffer strength.
ECR8404F Amino C6 Methacrylate allows multipoint covalent binding between enzymes and the resin. ECR8404F Amino C6 Methacrylate is produced via intense crosslinking in the presence of a porogenic agent that allows the calibration of porosity. The reacting amino group is on a long hexamethylene spacer that confers high flexibility to the immobilized enzyme.
ECR8404F Amino C6 Methacrylate is mechanically very stable and the final immobilized biocatalysts can be used in both stirred tank or bed reactor.
ECR8404F is compliant with Resolution ResAP 2004 (3) on ion exchange and adsorbent resins used in the processing of foodstuffs.
Application - Amino C6 Methacrylate - Covalent immobilization of enzymes
Polymer Structure - Acrylic
Appearance - Spherical Beads
Functional Group - Amino
Ionic Form as Shipped - Not Applicable
This product is a direct placement for ECR8405F. Changes have been made to improve the mechanical stability and purity.
|Particle Size Range||150 - 300 µm|
|Surface Area||> 40 m2/g|
|Pore Diameter Å||450 - 850|
|pH limits, Stability||3 - 10|
|For prolonged stability store dry between 2°C - 20°C|
The M grade version of this resin, with a diameter of 300-700 uM, is available Cat. #50262.
For more details download the Product Guide