Fast blue (FB) is a fluorescent dye most commonly used as a retrograde neuronal tracer. FB is the tracer of choice for motoneuronal labeling in long-term experiments as it is effectively transported retrogradely over long distances in various animal models, e.g. rats, cats and monkeys.
Fast Blue can be used alone or with other types of fluorescent retrograde and anterograde tracer dyes to develop picture of neuronal pathway. Other neuronal tracers such as Diamidino Yellow (DY), True Blue (TB), Granular Blue (GB), Evans Blue and Nuclear Yellow are used in conjunction with FB.
FB and DY are the most commonly used tracers for single or double retrograde fluorescent labeling. These dyes can be injected into laboratory animals at a concentration of 2-5% w/v in water. Common protocols recommend injecting 1-2 microliters of the 5.0% concentration into the desired area of the laboratory animal.
These protocols are offered as guides. Depending on the situation alternations may be needed due to the laboratory animal or research being done.
- Excitation Wavelength: 365nm
- Emission Wavelength: 420nm
- Soluble In:
- water, lower alcohols
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- Glove, chemical goggles & chemical mask or hood
- Store at room temperature