A sodium azide free buffer comprised of a neutral pH-buffered saline (i.e., Dulbecco's Phosphate-Buffered Saline) that contains 1% w/v paraformaldehyde. Sodium Azide –Free. Do not add sodium azide to buffers if you are concerned with recovering cell function.
e.g. if cells are to be collected for functional assays. Sodium azide inhibits metabolic activity no sodium azide in formula, this is kept separate to increase shelf life. Once mixed and aliquoted you may freeze. Thawed aliquots are stable at 4º C for up to two weeks, Aliquots good for at least 5 years. You may aliquot solution and sterilize them by autoclaving at 20 minutes, 121ºC, liquid cycle, in order to store at room temperature. You may store frozen aliquots for up to 5 years.
- Lanier LL, Warner NL. Paraformaldehyde Fixation of Hematopoietic Cells for Quantitative Flow Cytometry (FACS) Analysis. J Immunol Meth. 1981;47:25
- Harmless-use normal precautions
- Exercise normal care
- Store at 4 degrees celsius