Cell Culture Water is used for the preparation of cell culture media and related reagents. Our high quality water is produced to the strictest of standards and provides comparable performance to competitors' water.
Procedure: Three vials of CHO cells were thawed into 10 mL of CHO media formulated using three sources of water. Cells were grown for five days. Solutions were then normalized to 60 mL volume with density 5 x 105 cells per mL. The 60 mL cell suspensions were divided equally into 12 mL erylenmeyer shake flasks and incubated for three days (100 rpm shaker, 37°C, 95:5 air/CO2). Cell viability counts determined with trypan blue exclusion method on hemacytometer.