目录产品

Transporter™ 5 Transfection Reagent

View pricing for: Americas/Asia, Europe, Taiwan
Catalog No. Packaging Size 价格 数量
26008-1 1 mL
NT$2,352.00

描述

Transporter™ 5 Transfection Reagent is the ready-to-use form of a Polysciences proprietary linear polyethylenimine (PEI) derivative.  This ready-to-use transfection reagent condenses DNA into positively charged complexes which readily enter the cell by endocytosis. The Transporter™ 5-DNA complex is highly resistant to endosomal degradation, allowing high transfection efficiency.

Transporter™ 5 transfection reagent effectively introduces DNA into HEK-293, CHO, COS, HeLa, insect (Sf9 and Sf21) and other eukaryotic cell lines. Transporter™ 5 is most compatible with plasmids up to 135,000 nucleotides in size, although larger plasmids can be used with some success.
Appearance:
Liquid
Reference(s):

Rajendra, Y., Kiseljak, D., Baldi, L., Wurm, F. M. and Hacker, D. L. (2015), Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cellsBiotechnol Progress, 31: 541–549. doi: 10.1002/btpr.2064

Longo, P. a, Kavran, J. M., Kim, M. & Leahy, D. J. Transient Mammalian Cell Transfection wtih Polyethylenimine (PEI). Methods Enzymology 529, 227–240 (2013). doi:10.1016/B978-0-12-418687-3.00018-5.

Backliwal, G., Hildinger, M., Hasija, V. and Wurm, F. M. (2008), High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI. Biotechnol. Bioeng., 99: 721–727. doi: 10.1002/bit.21596

Tom, R., Bisson, L. & Durocher, Y. Transfection of HEK293-EBNA1 cells in suspension with linear PEI for production of recombinant proteinsCold Spring Harbor Protocols 3, 1–5 (2008). doi:10.1101/pdb.prot4977

Choosakoonkriang, S., Lobo, B. A., Koe, G. S., Koe, J. G. and Middaugh, C. R. (2003), Biophysical characterization of PEI/DNA complexes. J. Pharm. Sci., 92: 1710–1722. doi: 10.1002/jps.10437

Expired Patent: US6013240; Nucleic Acid Containing Composition, Preparation, and Uses of the Same


Hazards:
Harmless, use normal precautions
Handling:
Gloves & chemical googles
Storage:
Store at 4 degree C

MSDS / Technical Data Sheets / Product Literature

RELATED PRODUCTS