Transporter™ 5 Transfection Reagent is the ready-to-use form of a Polysciences proprietary linear polyethylenimine (PEI) derivative. This ready-to-use transfection reagent condenses DNA into positively charged complexes which readily enter the cell by endocytosis. The Transporter™ 5-DNA complex is highly resistant to endosomal degradation, allowing high transfection efficiency.Transporter™ 5 transfection reagent effectively introduces DNA into HEK-293, CHO, COS, HeLa, insect (Sf9 and Sf21) and other eukaryotic cell lines. Transporter™ 5 is most compatible with plasmids up to 135,000 nucleotides in size, although larger plasmids can be used with some success.
Rajendra, Y., Kiseljak, D., Baldi, L., Wurm, F. M. and Hacker, D. L. (2015), Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells. Biotechnol Progress, 31: 541–549. doi: 10.1002/btpr.2064
Longo, P. A., Kavran, J. M., Kim, M. & Leahy, D. J. Transient Mammalian Cell Transfection wtih Polyethylenimine (PEI). Methods Enzymology 529, 227–240 (2013). doi:10.1016/B978-0-12-418687-3.00018-5.
Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003
Backliwal, G., Hildinger, M., Hasija, V. and Wurm, F. M. (2008), High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI. Biotechnol. Bioeng., 99: 721–727. doi: 10.1002/bit.21596
Backliwal, G. et al. Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions. Nucleic Acids Research 36, e96–e96 (2008). doi:10.1093/nar/gkn423
Tom, R., Bisson, L. & Durocher, Y. Transfection of HEK293-EBNA1 cells in suspension with linear PEI for production of recombinant proteins. Cold Spring Harbor Protocols 3, 1–5 (2008). doi:10.1101/pdb.prot4977
Choosakoonkriang, S., Lobo, B. A., Koe, G. S., Koe, J. G. and Middaugh, C. R. (2003), Biophysical characterization of PEI/DNA complexes. J. Pharm. Sci., 92: 1710–1722. doi: 10.1002/jps.10437
Expired Patent: US6013240; Nucleic Acid Containing Composition, Preparation, and Uses of the Same
- Harmless, use normal precautions
- Gloves & chemical googles
- Store at 4 degree C; do not freeze. Shipped at room temperature (RT).