Diamidino Yellow (DY) is a fluorescent retrograde tracer used in experimental studies of the peripheral nervous system to determine the number and origin of neurons projecting to a specific area. The retrograde tracing techniques allow for detailed assessment of neuronal connections from a single population of neurons to their various targets throughout the nervous system. These techniques allow the "mapping" of connections between neurons in a particular structure (e.g. the eye) and the target neurons in the brain.
DY is transported over long distances in rats and cats, and produces a yellow fluorescence of the neuronal nucleus. When combined with True Blue (TB) or Fast Blue (FB), DY is effective in double labeling experiments aimed at demonstrating the existence of divergent axon collaterals.Excitation: 365 nm
Emission: >500 nm
- 88746-72-9 (Free Base) ; 87397-07-7 (2HCl)
- Soluble In:
Christiansen, K. et al. Complementary subicular pathways to the anterior thalamic nuclei and mammillary bodies in the rat and macaque monkey brain. The European journal of neuroscience(2016). doi:10.1111/ejn.13208
Wouterlood, F. G. A survey of current neuroanatomical tracing techniques. Neural Tracing Methods: Tracing Neurons and Their Connection. Neuromethods 92, 1–49 (2014). doi:10.1007/978-1-4939-1963-5_1
Akinori Ishikawa, Shoji Nakamura Ventral Hippocampal Neurons Project Axons Simultaneously to the Medial Prefrontal Cortex and Amygdala in the Rat J Neurophysiol 96, 2134-2138 (2006). DOI:10.1152/jn.00069.2006
Puigdellívol-Sánchez, A., Prats-Galino, A., Ruano-Gil, D. & Molander, C. Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: Efficacy of combined nerve injection and capsule application to transected nerves in the adult rat. Journal of Neuroscience Methods 95, 103–110 (2000). doi:10.1016/S0165-0270(99)00164-8
Skirboll, L. et al. A method for specific transmitter identification of retrogradely labeled neurons: Immunofluorescence combined with fluorescence tracing. Brain Research Reviews 8, 99–127 (1984). doi:10.1016/0165-0173(84)90001-8
Kuypers, H.G.J.M., Huisman, A.M. Fluorescent neuronal tracers, Adv. Cellular. Neurobiol. 5, 307-340 (1984).
Keizer, K., Kuypers, H. G. J. M., Huisman, A. M. & Dann, O. Diamidino yellow dihydrochloride (DY.2HCl); a new fluorescent retrograde neuronal tracer, which migrates only very slowly out of the cell. Experimental Brain Research 51, 179-191 (1983). doi:10.1007/BF00237193
- Store at Room Temperature