Sudan Black B is useful for staining neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and melting point 120-124 °C. It stains blue-black. Sudan Black B is one of the dyes used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan IV.
Color Index Number: 26150
Grade: Certified Biological StainUV Absorption: λmax 415nm (2nd), λmax 598nm
- Molecular Weight:
Subramaniam, H. N. & Chaubal, K. A. Evaluation of intracellular lipids by standardized staining with a Sudan black B fraction. Journal of Biochemical and Biophysical Methods 21, 9–16 (1990). doi:10.1016/0165-022X(90)90040-J
Penney, D. P., Powers, J. M., Frank, M., Willis, C. & Churukian, C. Analysis and testing of biological stains-- The Biological Stain Commission Procedures. Biotechnic & Histochemistry 77, 237–275 (2002). doi:10.1080/bih.77.5-6.237.275