A sodium azide free buffer comprised of a neutral pH-buffered saline (i.e., Dulbecco's Phosphate-Buffered Saline) that contains 1% w/v paraformaldehyde, sodium azide free.
Do not add sodium azide to buffers if you are interested in recovering cell function, e.g. if cells are to be collected for functional assays. Sodium azide inhibits metabolic activity. You may freeze the aliquoted mixture. Thawed aliquots are stable at room temperature for up to two weeks. You may sterilize aliquoted solution by autoclaving for 20 minutes at 121ºC liquid cycle to store at room temperature. Frozen aliquots is good for at least 5 years.
Lanier LL, Warner NL. Paraformaldehyde Fixation of Hematopoietic Cells for Quantitative Flow Cytometry (FACS) Analysis. J Immunol Meth. 1981;47:25
- Harmless-use normal precautions
- Exercise normal care