Lowicryls are low viscosity, highly cross-linked acrylate and methacrylate based embedding media. They are designed to preserve molecular structure/antigens by being applied at low temperatures. Because they preserve antigenicity, Lowicryls are a useful alternative to epoxy resins in immunochemistry.
Lowicryl® K kits are polar and hydrophilic and have many advantages over other methods. During dehydration and infiltration specimens may be kept partially hydrated and polymerized with up to 5% water. They are also useful in immunolabeling of sections using specific antisera, lectins and colloidal gold particles.
Lowicryl® K4M kit can be used for tissue infiltration and polymerization at temperature as low as −35ºC. The use of K4M results in better structural/antigen preservation and lower background labeling compared to other methods.
Kit contains: 1x130gm of Crosslinker A, 3x250gm of Monomer B, and 2x4.8gm of Initiator C
Dittmann C, Han HM, Grabenbauer M, Laue M. (2015). Dormant Bacillus spores protect their DNA in crystalline nucleoids against environmental stress. J Struct Biol. 191(2):156-64.
Newman GR, Hobot JA. (1999). Resins for combined light and electron microscopy: a half century of development. Histochem J. 31(8):495-505.
Altman LG, Schneider BG, Papermaster DS. (1984). Rapid embedding of tissues in Lowicryl K4M for immunoelectron microscopy. J Histochem Cytochem. 32(11):1217-23.
- Irritant, Lachrymator, Poison (Absorption)
- Glove, chemical goggles & chemical mask or hood
- Protect from light;Store at room temperature
- HIR7ag - (hazard/handling/storage codes)