This is a combined method utilizing the properties of both PAS and Alcian blue pH 2.5 methods to demonstrate the full complement of tissue proteoglycans. Alcian Blue pH 2.5 stains the acid mucins blue while PAS stains the neutral mucins pink to red.
- Acid mucins- blue
- Neutral mucins and other PAS positive tissue elements- pink to red
- Acid and neutral mucins mixtures- purple
Kit Contains: 500ml each: Alcian Blue (1% in 3% Acetic Acid), Schiff's Reagent, 0.5% Periodic Acid, Harris Hematoxylin, Scott's Bluing