Picrosirius Red Stain binds specifically to collagen fibrils of varying diameter that is used to distinguish collagen Type I from Type III. Picrosirius Red Stain will quantify the amount of collagen in a given area of myocardial tissue. Picrosirius Red staining is also useful in the quantitative aspect of liver fibrosis by measuring a change in collagen proportion stained as red (Jang et.al., 2014).
Solution A - Phosphomolybdic Acid (250ml or 500ml)
Solution B - Picrosirius Red F3BA Stain (250ml or 500ml)
Solution C - 0.1 N Hydrochloride Acid (250ml or 500ml)
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Jang, Y. O. et al. Histological improvement following administration of autologous bone marrow-derived mesenchymal stem cells for alcoholic cirrhosis: a pilot study. Liver International 34, 33–41 (2014). doi:10.1111/liv.12218
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- Gloves, chemical goggles
- Store at room temperature