Prussian Blue or Perls’ reaction is used to demonstrate ferric iron and ferritin. This is not a true staining technique rather, it is a histochemical reaction. The protein is split off by the hydrochloric acid, allowing the potassium ferrocyanide to combine with the ferric iron. This forms the ferric ferrocyanide or Prussian Blue.
Fixation: 10% NBF or alcohol can be used. Sections should be cut at 4μ to 5μ or blood or bone marrow smears.
Deparaffinize: Slides should be deparaffinized through xylene or substitute to remove paraffin from the section and descending grades of alcohol to water just prior to staining.
Avilés MO, Mangual JO, Ebner AD, Ritter JA. (2008) Isolated swine heart ventricle perfusion model for implant assisted-magnetic drug targeting. Int J Pharm. 2008 361(1-2):202-8.
Arbab AS, Bashaw, LA, Miller BR, Jordan EK, Lewis, BK, Kalish H, Frank JA Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging RSNA Radiology 2003 229(3).
- Gloves & chemical goggles
- Store at room temperature
- H4g - (hazard/handling/storage codes)