The dye exclusion test is used to determine the number of viable cells present in a cell suspension. It is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not. In this test, a cell suspension is simply mixed with dye and then visually examined to determine whether cells take up or exclude dye. In the protocol presented here, a viable cell will have a clear cytoplasm whereas a nonviable cell will have a blue cytoplasm.
This product is for research use only and is not intended for use in humans or for in vitro diagnostic use.
Strober, W. 1997. Current Protocols in Cytometry. A.3B.1-A.3B.2.
- Sodium azide may react with lead and copper plumbing to form explosive metal azides. Upon disposal of material, flush with a large volume of water to prevent azide accumulation. Please consult the Safety Data Sheet for more information.
- Containers which are opened must be carefully resealed and kept upright to prevent leakage.
- Keep container tightly closed in a dry, cool and well-ventilated place. Store at room temperature.