Linear polyethylenimine (PEI 25000) is a high-charge cationic polymer that readily binds highly anionic substrates. Industrially, linear PEI can improve the appearance of negatively charged dyes by modulating their properties and improving their adherence to surfaces. In research, PEI readily binds to DNA and other negatively charged biological molecules, making it one of the most efficient vector carriers available.
Up to 11% of the amine remains acylated (N-propionylated) from isomerization-polymerization of 2-ethyl-2-oxazoline followed by acid-hydrolysis of resulting N-propionyl-PEI. For fully deacylated PEI, see Polyethylenimine HCl MAX, Linear, Mw 40,000 (PEI MAX 40000; Catalog # 24765)
For ready-to-use PEI derivative, see Transporter™ 5 Transfection Reagent, Catalog # 26008 .
- 9002-98-6, 26913-06-4
- Molecular Weight:
- Melting Point:
- Soluble In:
- hot water, cold water at low pH, methanol, and ethanol.
Insoluble In: benzene, ethyl ether and acetone
- White to yellow solid
Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta Biomater. in Press, doi:10.1016/j.actbio.2015.09.020
Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002
Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties. Biomacromolecules. 6(6):3440-8.
Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.
- Gloves & fume hood
- Store at room temperature
- U5g - (hazard/handling/storage codes)