Linear polyethylenimine (PEI 25000) is a high-charge cationic polymer that readily binds with highly anionic substrates. Industrially, linear PEI can improve the appearance of negatively charged dyes by modulating their properties and improving their adherence to surfaces. In research, PEI readily binds to DNA and other negatively charged biological molecules, making it one of the most efficient vector carrier available.
PEI 25000 has 3-7% of the acylated amines (N-propionylated) from isomerization-polymerization of 2-ethyl-2-oxazoline followed by acid-hydrolysis of resulting N-propionyl-PEI. For fully deacylated PEI, see Polyethylenimine HCl MAX, Linear, Mw 40,000 (PEI MAX 40000; Catalog # 24765)
For ready-to-use PEI derivative, see Transporter™ 5 Transfection Reagent, Catalog # 26008 .
- 9002-98-6, 26913-06-4
- Molecular Weight:
- Melting Point:
- Soluble In:
- hot water, cold water at low pH, methanol, and ethanol.
Insoluble In: benzene, ethyl ether and acetone
- White to yellow solid
Kübelbeck, S., Mikhael, J., Schoof, S., Andrieu-Brunsen, A. & Baier, G. Immobilization of α-amylase in polyelectrolyte complexes. Journal of Applied Polymer Science In Press (2017). at <http://doi.wiley.com/10.1002/app.45036> doi:10.1002/app.45036
Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta Biomater. in Press, doi:10.1016/j.actbio.2015.09.020
Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002
Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003
Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.
Durocher, Y., Perret, S. & Kamen, A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research 30, E9 (2002). doi:10.1093/nar/30.2.e9
- Gloves & fume hood
- Store at room temperature