Gram's Decolorizer is used in the identification of Gram Positive and Gram Negative organisms. Consist of a 50/50 composition of alcohol to acetone in 4x16 oz replacement bottles. The decolorizer dissolves the lipids in the cell walls of Gram Negative organisms causing the crystal violet-iodine complex to rinse out. Gram Positive organisms resist this and hold onto the crystal violet-iodine complex. FDA approved for in vitro diagnostic use.