Uvitex 2B

Uvitex 2B fluorescent dye will bind to the chitin in fungal walls and is highly selective for fungi and algae in tissue sections. It has a slower fade rate than more commonly used fungal fluorescent dyes.

(C.I. Fluorescent Brightener 362; Derivative of stilbene disulfonic acid.)

Commonly used protocol for staining of Microsporidia and Blastomyces is as follows:

1% Uvitex 2B in Phosphate Buffered Saline or 0.1% Uvitex 2B in 0.1M Tris-HCl Buffer (pH 5.8)

Stain for 10-30 minutes

Rinse with saline solution

Counterstain with Gill's Hematoxylin #2

Dehydrate, clear and mount specimen

Excitation Max: 350nm

Emission Max: 435nm in PBS Buffer