HOPE® - Formalin-free fixation technique for molecular pathology and research!
Tissue conservation using the new HOPE®-technique provides paraffin embedded tissue blocks with native structure of the macro-molecules (protein, DNA, RNA) in the tissue.
During HOPE® fixation, tissue samples are incubated in HOPE® I solution, then in HOPE® II solution, followed by a dehydration with acetone and subsequent embedding in paraffin. For this tissue processing no alcohols or aldehydes (formalin, glutaraldehyde) are used. As a consequence, there is no cross-linking of macro- molecular structures during the process. This opens up new opportunities to characterize tissues out of paraffin blocks.
Applications for immunohistochemistry, in situ hybridization targeting RNA and DNA, PCR and RT-PCR have been demonstrated using properly prepared paraffin embedded tissue sections. Tissue specimens fixed with the HOPE® technique allow greater morphology and retrospective studies on the molecular level.
The HOPE®-Fixation technique requires HOPE® Fixative System II and HOPE® Fixative System I to complete the protocol.
- In situ hybridization targeting RNA and DNA
- Achieve IHC on paraffin tissue without antigen unmasking
- IHC using antibodies which do not work on formalin fixed parafffin embedded tissue
- No more overfixed formalin tissue samples
- Unmatched results in RNA and DNA in situ hybridization
- Excellent conservation of nucleic acids for PCR and RT-PCR