Transporter™ 5 Transfection Reagent

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26008-1 1 mL
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26008-5 5 x 1 mL
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Transporter™ 5 transfection reagent is the ready-to-use form of a proprietary linear polyethylenimine (PEI) derivative.  Transporter™ 5 condenses DNA into positively charged complexes which readily enter the cell by endocytosis. The Transporter™ 5-DNA complex is highly resistant to endosomal degradation, allowing high transfection efficiency.

Transporter™ 5 transfection reagent effectively introduces DNA into HEK-293, CHO, COS, HeLa, insect (Sf9 and Sf21) and other eukaryotic cell lines. Transporter™ 5 is most compatible with plasmids up to 135,000 nucleotides in size, although larger plasmids can be used with some success.


Longo, P. a, Kavran, J. M., Kim, M. & Leahy, D. J. Transient Mammalian Cell Transfection wtih Polyethylenimine (PEI). Methods Enzymology 529, 227–240 (2013). doi:10.1016/B978-0-12-418687-3.00018-5.

Rajendra, Y., Kiseljak, D., Baldi, L., Wurm, F. M. and Hacker, D. L. (2015), Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells. Biotechnol Progress, 31: 541–549. doi: 10.1002/btpr.2064

Backliwal, G., Hildinger, M., Hasija, V. and Wurm, F. M. (2008), High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI. Biotechnol. Bioeng., 99: 721–727. doi: 10.1002/bit.21596

Choosakoonkriang, S., Lobo, B. A., Koe, G. S., Koe, J. G. and Middaugh, C. R. (2003), Biophysical characterization of PEI/DNA complexes. J. Pharm. Sci., 92: 1710–1722. doi: 10.1002/jps.10437

Expired U.S. Patent No. 6,013,240; Nucleic Acid Containing Composition, Preparation, and Uses of the Same

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