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Specific conductance of 35% (w/v) solution 2µmho/cm. Used in electrophoresis for separation of nucleic acid fragments and proteins. For precision gels used in electro-phoresis and isoelectric focusing. Because of the low acidity levels of our acrylamide, binding of ampholytes is virtually eliminated and fine pH gradients can be established. Fluorescence-free gels are readily prepared. For scanning of gels n the UV region, our Ultra Pure grade acrylamide makes gels with baselines having very low "noise".
Silver staining is a highly sensitive method for detecting proteins in polyacrylamide slab gels. Most silver staining protocols are time consuming, complicated, and dependent upon the purity of the reagents. Polysciences’ Silver Stain kit is simple, stable, controllable, and very rapid. Our method is sensitive to proteins in the nanogram range and may be used either before or after Coomassie blue staining. Staining for proteins is initiated in an alkali environment.Learn More