Granular Blue (GB) Dihydrochloride

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Catalog No. Packaging Size Price Quantity
26273-1 1 mg
26273-5 5 mg


Granular Blue (GB) is a fluorescent retrograde tracer used in experimental studies of the peripheral nervous system to determine the number and origin of neurons projecting to a specific area. The retrograde tracing techniques allow for detailed assessment of neuronal connections from a single population of neurons to their various targets throughout the nervous system. These techniques allow the "mapping" of connections between neurons in a particular structure (e.g. the eye) and the target neurons in the brain.

In rats, GB is effectively transported over long distances to produce blue fluorescent labeling of neuronal cytoplasm in cell bodies and proximal dendrites. It also produces an accumulation of silver-blue granules in cytoplasm.

Excitation: 365 nm
Emission: 410 nm

C22H19N5O · 2HCl

Molecular Weight:
Soluble In:


Liang, Y.-X. et al. Uptake of Retrograde Tracers by Intact Optic Nerve Axons: A New Way to Label Retinal Ganglion Cells. PLOS ONE 10, e0128718 (2015). doi:10.1371/journal.pone.0128718

Wouterlood, F. G. A survey of current neuroanatomical tracing techniques. Neural Tracing Methods: Tracing Neurons and Their Connection. Neuromethods 92, 1–49 (2014). doi:10.1007/978-1-4939-1963-5_1

Distler, C., Mustari, M. J. & Hoffmann, K.-P. Cortical projections to the nucleus of the optic tract and dorsal terminal nucleus and to the dorsolateral pontine nucleus in macaques: A dual retrograde tracing study. The Journal of Comparative Neurology 444, 144–158 (2002). doi:10.1002/cne.10127

Harmann, P. A., Carlton, S. M. & Willis, W. D. Collaterals of spinothalamic tract cells to the periaqueductal gray: a fluorescent double-labeling study in the rat. Brain Research 441, 87–97 (1988). doi:10.1016/0006-8993(88)91386-8

Kuypers, H.G.J.M., Huisman, A.M. Fluorescent neuronal tracersAdv. Cellular. Neurobiol. 5, 307-340 (1984).

Store at room temperature

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