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Uvitex 2B

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19517-10 10 g
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Description

Uvitex 2B fluorescent dye will bind to the chitin in fungal walls and is highly selective for fungi and algae in tissue sections. It has a slower fade rate than more commonly used fungal fluorescent dyes.

(C.I. Fluorescent Brightener 362; Derivative of stilbene disulfonic acid.)

Commonly used protocol for staining of Microsporidia and Blastomyces is as follows:

    1% Uvitex 2B in Phosphate Buffered Saline or 0.1% Uvitex 2B in 0.1M Tris-HCl Buffer (pH 5.8)
    Stain for 10-30 minutes
    Rinse with saline solution
    Counterstain with Gill's Hematoxylin #2
    Dehydrate, clear and mount specimen

Excitation Max: 350nm

Emission Max: 435nm in PBS Buffer

CAS#:
27344-41-8
Reference(s):

Lab. Med., 18, 603 (1987)

J Clinical Path 1993;46:694-699


TSCA
 
Hazards:
Corrosive , Irritant
Handling:
Glove, chemical goggles & fume hood
Storage:
Store at room temperature
Code:
BH6g - (hazard/handling/storage codes)

MSDS / Technical Data Sheets / Product Literature