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A STAT staining method for unfixed and fixed tissue. Stains nuclei more strongly than cell cytoplasm.

Excellent dye for nuclear and nucleolar details. Can be used to demonstrate erythrocyte alterations/inclusions and some erythrocyte parasites, as well as to visualize reticulocytes. Methylene blue is different from that in Romanowsky stains. In order to stain a slide with methylene blue a drop of stain is placed on a coverslip. The coverslip is then placed on an unfixed blood smear. An alternative method is to mix some stain with fresh blood before a blood smear is made.