Carboxyl (COOH) microparticles can be used for covalent coupling of proteins by activating the carboxyl groups with water-soluble carbodiimide. The carbodiimide reacts with the carboxyl group to create an active ester that is reactive toward primary amines on the protein of interest.
The PolyLink Protein Coupling Kit contains buffers and carbodiimide (EDAC) derivative for coupling proteins to carboxylated beads. The contents of the kit are sufficient for 50 coupling reactions using 200-500µg of protein per reaction. The kit has been optimized using purified IgG as the coupling protein.
Simple protocol with easy to follow instruction optimized for polymer microspheres 1µm or larger are included. For microspheres less than 0.1µm, see our Vivaspin® Ultrafiltration Devices (cat code BLIAA022)
Ross, D. & Altmann, M. A fast protocol for purification of translating mRNAs. Methods, (2016) in press. doi:10.1016/j.ymeth.2016.03.021
Halbeisen, R. E., Scherrer, T. & Gerber, A. P. Affinity purification of ribosomes to access the translatome. Methods 48, 306–310 (2009). doi:10.1016/j.ymeth.2009.04.003
Ip, W. K. E., Takahashi, K., Moore, K. J., Stuart, L. M. & Ezekowitz, R. A. B. Mannose-binding lectin enhances Toll-like receptors 2 and 6 signaling from the phagosome. The Journal of Experimental Medicine 205, 169–181 (2008). doi:10.1084/jem.20071164
, Skin sensitizer
- Glove, chemical goggles & fume hood
- Protect from moisture;Store below -20 degrees celsius
- HO6bf - (hazard/handling/storage codes)