Nile Blue Methacrylamide

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Catalog No. Packaging Size Price Quantity
25355-100 100 mg


Nile Blue dye itself is a fluorescent dye that is used routinely in histology to impart a blue color to cell nuclei, where it highlights the distinction between neutral lipids (triglycerides, etc.) which are stained pink and fatty acids, which are stained blue.

Nile Blue Methacrylamide has been used to covalently link Nile Blue dye into the backbone of polymers for use in various sensor applications. Chandrasekharan, et al have developed a plasticizer-free autoclavable ratiometric alcohol sensor based on Nile Blue Methacrylamide incorporated into a hydrogel polymer backbone.1 The film formed from the Nile Blue hydrogel exhibits both absorption and fluorescence changes upon contact with ethanol, and the fluorescence λ max decreases with the increase of ethanol concentration. The sensor can therefore be used to accurately determine ethanol concentration in a system.

In addition, Pham, et al have developed a polymeric nanostructured material incorporating Nile Blue methacrylamide into the polymer, and this material is being developed for the recording of biometric features for anti-counterfeiting applications.2

Purity: >85% (HPLC relative absorption from λ=254 nm); ca. 90% based on 1H NMR

UV Absorption and fluorescence emission maxima of Nile Blue in different solvents3

Solvent Absorption λmax(nm) Emission λmax(nm)
Toluene 493 574
Acetone 499 596
DMF 504 598
Ethanol 628 667
Water 635 674
1.0 N HCI (pH=1.0) 457 556
0.1 N NaOH (pH=11.0) 522 668

* Excitation and emission data is for Nile Blue dye itself.

Store: in dark at 0º C



Molecular Weight:
Dark Purple Crystals
1. Chandrasekharan, N.; Ibrahim, S.; Kostov, Y.; Rao, G. Bioautomation 9 (2008) 31-39. 2. Pham, H., et al J. Mater. Chem. 17 (2007) 523-526. 3. J. Jose; K. Burgess Tetrahedron 62 (2006) 11021-11037
Protect from light;Store below 0 degrees celsius

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