Linear polyethylenimine HCl MAX, Mw 40,000 (PEI MAX 40000) is a fully hydrolyzed (deacylated), highly water soluble hydrochloride salt form of our linear polyethylenimine, Mw 25,000 (PEI 25000, Cat. # 23966). PEI MAX 40000 contains a longer contiguous ethyleneimine segments resulting in more than 11% increase in protonable nitrogens than PEI 25000.
PEI MAX 40000 is supplied as a hydrochloride salt for ease of handling and high water solubility. PEI MAX 40000 can be converted into the linear free amine (PEI 22000) form by neutralization with base.
For our ready-to-use PEI derivative, see Transporter™ 5 Transfection Reagent, Catalog # 26008 .
- 26338-45-4, 9002-98-6, 26913-06-4
- Molecular Weight:
- 40,000 (~22,000 free base)
- Soluble In:
- Cold and room temperature water
Insoluble in: Common organic solvents (ethanol, acetone, tetrahydrofuran)
- White to off-white free flowing solid
Longo, P. a, Kavran, J. M., Kim, M. & Leahy, D. J. Transient Mammalian Cell Transfection wtih Polyethylenimine (PEI). Methods Enzymology 529, 227–240 (2013). doi:10.1016/B978-0-12-418687-3.00018-5.
Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002
Mann JF, McKay PF, Arokiasamy S, Patel RK, Klein K, Shattock RJ. (2013). Pulmonary delivery of DNA vaccine constructs using deacylated PEI elicits immune responses and protects against viral challenge infection. J Control Release. 170(3):452-9.
Kami, D. et al. Application of magnetic nanoparticles to gene delivery. International Journal of Molecular Sciences 12, 3705–3722 (2011). doi:10.3390/ijms12063705
Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.
- Glove, chemical goggles & fume hood
- Store at room temperature