5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride (CTC)
Cyanoditolyl tetrazolium chloride has been used to measure the redox activity of tumor cells. It has also been employed for direct epifluorescent microscopic enumeration of respiring bacteria in food samples and environmental samples, especially water samples. Reveals a quantitative methodology for measuring marine bacteria. These methods yield 80% activity in 2 to 10 minutes. Cell samples mixed with CTC can be stored refrigerated or frozen in liquid nitrogen for at least 4 weeks without a significant loss of cells.
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ME. Sieracki, TL. Cucci, and J. Nicinski Flow Cytometric Analysis of 5-Cyano-2,3- Ditolyl Tetrazolium Chloride Activity of Murine Bacterioplankton in Dilution Cultures Appl. Environ. Micro. June 1999, p. 2409-2417 Vol. 65 (6)
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