Formaldehyde, 10% Aqueous Solution, Methanol-Free (Ultra Pure)
This ultra-pure, methanol-free 10% aqueous formaldehyde solution is formulated for use in both light microscopy
and electron microscopy workflows. The solution readily penetrates large tissue blocks, supporting effective
fixation in a wide range of histological and ultrastructural applications.
When used in combination with glutaraldehyde, this formaldehyde solution is commonly applied to the fixation of
delicate tissues, such as brain tissue, including vascular perfusion protocols. Because it is supplied as a
ready-to-use aqueous solution, it eliminates the need for depolymerization of paraformaldehyde prior to use.
The formaldehyde is derived from paraformaldehyde and can be used as a component of Karnovsky-type fixatives in
conjunction with user-supplied buffer systems, depending on experimental requirements.
For storage, the solution should be kept tightly sealed at room temperature. Exposure to air may promote oxidation
of formaldehyde to formic acid, while cold storage can encourage formation of trioxymethylene as a white precipitate.
Key Properties
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Chemical type: Formaldehyde, aqueous solution
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Concentration: 10% (w/v equivalent)
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Methanol content: Methanol-free
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Purity: Ultra-pure grade
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Source material: Derived from paraformaldehyde
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Penetration: Effective penetration of large tissue blocks
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Format: Ready-to-use aqueous solution
Applications
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Light microscopy fixation for histological specimens
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Electron microscopy fixation and ultrastructural preservation
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Vascular perfusion fixation (including delicate tissues such as brain)
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Karnovsky-type fixative preparation with user-defined buffer systems
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General laboratory tissue fixation where methanol-free formaldehyde is required
Storage and Handling
- Store tightly sealed at room temperature
- Avoid prolonged exposure to air to reduce oxidation to formic acid
- Avoid cold storage to minimize formation of trioxymethylene precipitate
FAQs
Common questions about Formaldehyde, 10%, Methanol-Free (Ultra Pure).
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What is this 10% formaldehyde solution used for?
It is commonly used as a tissue fixative for light microscopy and electron microscopy, supporting effective
preservation of cellular and tissue structure.
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Why is methanol-free formaldehyde important?
Methanol-free formulations are often selected to avoid potential artifacts or unwanted effects associated
with methanol stabilizers, depending on the application and downstream analysis.
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Can this product replace paraformaldehyde preparation?
Yes. Because it is supplied as a ready-to-use aqueous solution derived from paraformaldehyde, it eliminates
the need to depolymerize paraformaldehyde prior to fixation.
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Is this suitable for use in Karnovsky-type fixatives?
It is commonly used as a component of Karnovsky-type fixatives when combined with glutaraldehyde and an
appropriate buffer system selected by the user.
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How should this formaldehyde solution be stored?
It should be stored tightly sealed at room temperature. Exposure to air may promote oxidation to formic
acid, while cold storage can lead to formation of a white trioxymethylene precipitate.
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Is this solution suitable for delicate tissues such as brain?
When used alone or in combination with glutaraldehyde, it is commonly applied for fixation of delicate
tissues, including brain tissue, particularly in perfusion-based protocols.
