Sternberger’s peroxidase-anti-peroxidase (PAP) immunocytochemical staining technique employs linkage of the visualizable enzyme horseradish peroxidase through unlabeled antibodies to a tissue antigen. Thus, any antigen for which you can prepare an antiserum can be visualized at the EM or light microscope level. The multiplication factor that is characteristic of enzyme reactions makes this one of the most sensitive assay techniques available.
- L.A. Sternberger, “Immunocytochemistry,” Prentice Hall (1983)
- Gloves & chemical goggles
- Store at 4 degrees celsius