TB Fluorostain Kit
Fluorescent detection of M. tuberculosis and other acid-fast bacteria. Fluorochrome staining of mycobacteria in an acid-fast smear offers several advantages over traditional carbol-fuchsin methods. Lower magnification is required; Mycobacteria, including M. tuberculosis, M. leprae, M. avium-intracellular fluoresce orange-yellow on a dark black background. M. tuberculosis appears as slightly curved or straight rods with rounded ends. The rods will be 0.3 to 0.6μm in length.
Features:
- 100 tests per kit
- Less than 12 minutes to perform staining
- Microwave method reduces time and enhances results
- Much less Auramine O and Rhodamine B used, reducing costs and hazards
- Faster, superior counterstain
FDA approved for in vitro diagnostic use in the United States
Kit contains:
500ml of Staining Solution A
500ml of Acid Solution B
500ml Counterstaining Solution C
Borrmann, E., Mobius, P., Diller, R. & Kohler, H. Divergent cytokine responses of macrophages to Mycobacterium avium subsp. paratuberculosis strains of Types II and III in a standardized in vitro model. Veterinary Microbiology 152, 101–111 (2011). doi:10.1016/j.vetmic.2011.04.002
Jeyanathan, M., Alexander, D. C., Turenne, C. Y., Girard, C. & Behr, M. A. Evaluation of in situ methods used to detect Mycobacterium avium subsp. paratuberculosis in samples from patients with Crohn’s disease. Journal of clinical microbiology 44, 2942–50 (2006). doi:10.1128/JCM.00585-06
Converse, P. J. et al. Cavitary tuberculosis produced in rabbits by aerosolized virulent tubercle bacilli. Infection and Immunity 64, 4776–4787 (1996).
