Acridine orange, C.I. 46005 (min. 95%)

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Catalog No. Packaging Size Price Quantity
04539-1 1 g
04539-5 5 g


Acridine orange is a nucleic acid-selective fluorescent cationic dye useful for cell cycle determination. It emits green fluorescence (525 nm) when bound to dsDNA and red fluorescence (650 nm) when bound to ssDNA or RNA.

Polysciences' grade of acridine orange is of exceptionally high purity, suitable for quantitative work. It is free of inorganic salts. We offer a highly purified form of acridine orange while most of the other commercially available grades are either of low purity or in zinc chloride complex form.

3,6-Bis(dimethylamino)acridine hydrochloride hydrate 

Purity 95% (HPLC)
Molecular Weight:
301.83 (anhydrous)

Yagci, A., Murk, W., Stronk, J. & Huszar, G. Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange fluorescence study. Journal of andrology 31, 566–572 (2010). doi:10.2164/jandrol.109.008912

Paulsen, R. D. & Nettleton, G. S. Analysis of commercial samples of acridine orange using high-performance liquid chromatography. The journal of histochemistry and cytochemistry 36, 1489–93 (1988). doi:10.1177/36.12.3192936

Gerdes, J. et al. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. Journal of immunology 133, 1710–5 (1984).

Mcmaster, G. K. & Carmichaeltt, G. G. Analysis of single-and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orangeProc. Natl. Acad. Scf. USA 74, 4835–4838 (1977).

Irritant , Unknown
Gloves & fume hood
Store below 0 degrees celsius

MSDS / Technical Data Sheets / Product Literature