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Bisbenzimide (Hoechst 33258)

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Catalog No. Packaging Size Price Quantity
09460-100 100 mg
€98.00

Description

Hoechst 33258 is a blue fluorescent dyes used to stain DNA. A concentration of 0.1-12 µg/ml is commonly used to stain DNA in bacteria or eukaryote cells. Cells are stained for 1-30 min (dependent on section thickness) at room temperature or 37 °C and then washed to remove unbound dye. Dye is excited by ultraviolet light at around 350 nm, and emits blue-cyan fluorescent light around an emission spectrum maximum at 461 nm.

This dye is used as substitutes for another nucleic acid stain called DAPI Catalog # 09224.
CAS#:
23491-45-4
Formula:

C27H37CI3N6O4


Molecular Weight:
623.97
Reference(s):

Science, 220, 620 (1983), Anal. Biochem., 179, 401 (1989)


Hazards:
Irritant
Handling:
Gloves & chemical goggles
Storage:
Protect from light;Protect from moisture;Store at 4 degrees celsius

MSDS / Technical Data Sheets / Product Literature