5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride (CTC)

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Catalog No. Packaging Size Price Quantity
19292-50 50 mg
19292-100 100 mg
19292-1 1 g


Cyanoditolyl tetrazolium chloride has been used to measure the redox activity of tumor cells. It has also been employed for direct epifluorescent microscopic enumeration of respiring bacteria in food samples and environmental samples, especially water samples. Reveals a quantitative methodology for measuring marine bacteria. These methods yield 80% activity in 2 to 10 minutes. Cell samples mixed with CTC can be stored refrigerated or frozen in liquid nitrogen for at least 4 weeks without a significant loss of cells.

Formazan fluoresces in solid state:
  • Exc max: 450nm
  • Emis max: 630nm
  • CAS#:
    Molecular Weight:
    Melting Point:
    Soluble In:
    ~100mg in 6.6cc water

    J.Stellmach and E. Severin A fluorescent redox dye Histochem. J. 19, 21 (1987)

    GG. Rodriguez, D. Phipps, K. Ishiguro and HF. Ridgeway Use of a fluorescent redox probe for direct visualization of actively respiring bacteria Appl. Environ. Microbiol., 58, 1801 (1992)

    BH. Pyle, SC. Broadaway Sensitive Detection of Escherichia coliO157: H7 in Food and Water by Immunomagnetic Separation and Solid-Phase Laser Cytometry Appli. Environ. Microbiol., 65(5), 1966 (1999)

    LJ. Rose, R.Donian, SN. Banerjee and MJ. Arduino Survival of Yersinia pestis on Environmental Surfaces Appl. Environ. Microbiol., 69(4), 2166 (2003)

    ME. Sieracki, TL. Cucci, and J. Nicinski Flow Cytometric Analysis of 5-Cyano-2,3- Ditolyl Tetrazolium Chloride Activity of Murine Bacterioplankton in Dilution Cultures Appl. Environ. Micro. June 1999, p. 2409-2417 Vol. 65 (6)

    Irritant , Unknown
    Glove, chemical goggles & fume hood
    Protect from light;Store at 4 degrees celsius

    MSDS / Technical Data Sheets / Product Literature