(Direct blue 14; Niagara blue 3B)
Trypan blue has been widely used to assess the viability of eukaryotic cells. This method is often called trypan blue exclusion. Nonviable cells will exhibit a marked concentration of this blue dye in their nuclei. Tissue culture labs will find it extremely useful for determining the health and density of cell lines. Other studies have demonstrated trypan blue as an inactivating agent for measles and herpes simplex viruses and as a teratogenic agent in rats.
λ max 607nm
- Molecular Weight:
- Melting Point:
Thorne, H. V. & Clarke, G. F. Inactivation of Measles and Herpes Simplex Viruses by Trypan Blue. Journal of General Virology 64, 1365–1368 (1983). doi:10.1099/0022-1317-64-6-1365
Gulamhusein, A. P., Moore, W. J., Gupta, M. & Beck, F. Trypan blue teratogenesis in the rat: Further observations in vitro. Teratology 26, 289–297 (1982). doi:10.1002/tera.1420260311
Jilka, R. L., Weinstein, R. S., Bellido, T., Parfitt, A. M. & Manolagas, S. C. Osteoblast programmed cell death (apoptosis): modulation by growth factors and cytokines. Journal of Bone and Mineral Research 13, 793–802 (1998). doi:10.1359/jbmr.19188.8.131.523
O’BRIEN, R. & GOTTLIEB-ROSENKRANTZ, P. AN AUTOMATIC METHOD FOR VIABILITY ASSAY OF CULTURED CELLS. THE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY 18, 581–589 (1970).
, Toxic (Ingestion)
, Toxic (Absorption)
, Toxic (Inhalation)
- Glove, chemical goggles & fume hood
- Store at room temperature