JB-4® is a water-soluble, 2-hydroxyethyl methacrylate (HEMA), more commonly known as "glycol methacrylate", or GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy (HRLM). JB-4 embedded tissues yield semi-thin sections (0.5µ-2µ) with excellent morphological structure preservation than paraffin processed tissues. A water-soluble media, JB-4 does not require dehydration to absolute alcohol except for dense, bloody, or fatty tissue specimens. Clearing agents such as xylene and chloroform are not required. JB-4 is excellent for small non-decalcified bone specimens, routine stains, special stains, and histochemical staining.
JB-4 is the leading water-soluble plastic embedding kit for light microscopy that is widely used for research and clinical diagnosis. The polymerization of JB-4 is exothermic, which is easily controlled by polymerizing on ice or by using refrigeration at 4°C. Clear casts are obtained in 90 minutes or less. It has been successfully used in enzyme histochemistry, and auto-radiography.
JB-4® Embedding Kit Contains: 800ml Solution A (Monomer) ; 30ml Solution B (Accelerator) ; 12g Catalyst (Benzoyl Peroxide, Plasticized).
For immuno procedures we recommend the Osteo-Bed Bone Embedding Kit (Catalog #17734). JB-4 is not recommended for immuno procedures as the glycol methacrylate cannot be removed from the section and may block antigen sites for most antibody reactions.
Sullivan-Brown J., Bisher M.E., Burdine R.D., (2011) Embedding, Serial Sectioning and Staining of Zebrafish Embryos Using JB-4™ Resin. Nature Protocols 6(1):46-55
Xiaofang Wang X., Johnson D.H., (2000) mRNA In Situ Hybridization of TIGR/MYOC in Human Trabecular Meshwork. Invest Ophthalmol Vis Sci 41:1724–1729
Duello T.M., Gumkowski F.C., (1986) Immunocytochemical localization of protein antigens in large sections of tissues embedded in water-soluble embedding media. J Histochem Cytochem 34(4): 535-538
Horton W.A., Dwyer C., Goering R., Dean D.C., (1983) Immunohistochemistry of types I and II collagen in undecalcified skeletal tissues. J Histochem Cytochem 31(3):417-425.
Tacha, D.E., Richard T.C., (1981) Comparison of Glycol Methacrylate-and Paraffin-Embedded Tissues for identification of Tritium-Labeled Cells after Emulsion Radioautography. J Histotech 4(2):59-62
Higuchi S., Suga M., Dannenberg A.M. Jr, Schofield B.H., (1979) Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections. Stain Technol, 54(1):5-12.
, Skin sensitizer
- Glove, chemical goggles & fume hood
- Store at 4 degrees celsius