Prussian Blue Iron Stain Kit (Reaction for Demonstration of Iron)

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Catalog No. Packaging Size Price Quantity
24199-1 1 kit


Prussian Blue or Perls’ reaction is used to demonstrate ferric iron and ferritin. This is not a true staining technique rather, it is a histochemical reaction. The protein is split off by the hydrochloric acid, allowing the potassium ferrocyanide to combine with the ferric iron. This forms the ferric ferrocyanide or Prussian Blue.

Fixation: 10% NBF or alcohol can be used. Sections should be cut at 4μ to 5μ or blood or bone marrow smears.

Kit contains: 250 ml of each: 4% Potassium Ferrocyanide, 4% Hydrochloric Acid, 1% Nuclear Fast Red

250 ml kit stains up to 125 slides

Balivada, S. et al. A/C magnetic hyperthermia of melanoma mediated by iron(0)/iron oxide core/shell magnetic nanoparticles: a mouse study. BMC Cancer 10, 119 (2010). doi:10.1186/1471-2407-10-119

Zhao, H., Wong, R. J., Kalish, F. S., Nayak, N. R. & Stevenson, D. K. Effect of Heme Oxygenase-1 Deficiency on Placental Development. Placenta 30, 861–868 (2009). doi:10.1016/J.PLACENTA.2009.07.012

Avilés MO, Mangual JO, Ebner AD, Ritter JA. (2008) Isolated swine heart ventricle perfusion model for implant assisted-magnetic drug targeting. Int J Pharm. 2008 361(1-2):202-8.

Arbab AS, Bashaw, LA, Miller BR, Jordan EK, Lewis, BK, Kalish H, Frank JA  Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging RSNA Radiology 2003 229(3).

Gloves & chemical goggles
Store at room temperature